QuickFISH™ Rapid Pathogen ID can help!
The ideal complement to MALDI-TOF
Although the MALDI-TOF system is becoming established as a technique of choice for routine use in the medical laboratory, nevertheless there are occasions where it may not provide a clear species identification.
In these circumstances, the MALDI-TOF data may need to be verified using a second identification method.
Situations that MALDI-TOF can find challenging include:
- gram positive bacteraemia
- presence of charcoal or resin in the culture
- polymicrobial bacteraemia
Thankfully the QuickFISH system is often able to identify pathogens directly from blood cultures when a MALDI-TOF system may encounter problems. This robust, reliable and sensitive system needs minimal sample preparation. It provides the ideal backup for MALDI-TOF to ensure that the laboratory can always quickly and accurately report findings across the broadest possible spectrum of pathogens.
Furthermore, the unmatched 20 minute turnaround time offered by QuickFISH allows a positive pathogen ID to be reported with the gram stain result at a critical antibiotic therapy decision point.
The QuickFISH system can rapidly and reliably provide positive identification of Staphylococci, Enterococci, gram-negative rods and yeasts, directly from positive blood cultures, without any need for extensive sample preparation or complex equipment.
As such it represents the ideal solution for a busy clinical microbiology laboratory with the need to optimise its procedures for handling positive blood cultures, while detecting the fullest range of pathogens.
The benefits of PNA-FISH and QuickFISH technology are becoming increasingly reported in the literature.
“PNA-FISH is easy to perform in the clinical laboratory and does not require significant
capital equipment costs unlike microarrays or MALDI-TOF”.
Harris DM, Hata DJ. AnnClin Microbiol Antimicrob 2013;12:2.
“The QuickFISH technology appears robust and reliable, with the major advantage of providing results in half an hour. It can be expected that this technique will be used routinely for all blood cultures”.
Caretto E, et al, J Clin Microbiol 2013;51:131-5.
“The method [QuickFISH] provides a presumptive identification of S. aureus and classifies most non-S. aureus staphylococci as CoNS. Clinical trial results demonstrated high sensitivity and specificity for detection of staphylococci in blood cultures & rapid & easy discrimination of S. aureus and CoNS”.
Deck et al, J Clin Microbiol 2012;50:1994-8
This article was published previously in Alpha Laboratories’ Leading Edge Newsletter – Autumn 2013.