Staphylococcus QuickFISH for Rapid Diagnosis of Sepsis from Blood Cultures

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Staphylococcus aureus bacteraemia is a common and very serious infection. S. aureus bacteraemia is associated with high rates of morbidity and mortality and its rapid and accurate diagnosis is vital. However the clinician must be able to distinguish between a bacteraemia caused by S.aureus, and bacteraemias caused by other Staphylococcus species (such as coagulase negative Staphylococci – CoNS) in order to choose the most appropriate therapy. In addition CoNS contaminants can also cause a ‘false positive’ diagnosis of S.aureus bacteraemia. As such accurate identification of Staphylococci to species level is extremely important.

Staphylococcus species look identical under the light microscope after gram staining, and further identification to species by culture methods can take 24-48 hours. As a precaution (in case the infection is due to S.aureus) Staphylococcal bacteraemias are frequently treated with vancomycin until a positive species ID becomes available. However as QuickFISH can distinguish between S.aureus and other Staphylococcus species in 20 minutes it can remove this requirement.

S. aureus
S. aureus
CoNS
CoNS
Negative result
Negative

Staphylococcus QuickFISH provides a positive, unambiguous species ID in 20 minutes, and is based on the proven, patented PNA-FISH technology from AdvanDX.

Identification of CoNS in 20 minutes rather than 24-48 hours could deliver:

  • 2 day reduction in length of hospital stay per patient with CoNS 1,2
  • 4.5 doses of Vancomycin saved per patient with CoNS 1

 

Identification of S.aureus in 20 minutes rather than 24-48 hours could deliver:

  • 53% reduction in patient mortality 2

 

Staphylococcus QuickFISH allows clinicians to rapidly ensure early, appropriate therapy for patients with S.aureus bacteraemia, while minimising the unnecessary use of resources and broad spectrum antibiotics on those with CoNS.

Performance Data

Please see Instructions for Use (IFU) for complete performance data.

Conventional Identification

S.aureus CoNS Other Species Total
QuickFISH S. aureus 150 0 1 151
CoNS 1 351 1 353
Negative 0 6 43 49
Total 151 357 45 553

 

Key Benefits at a Glance

  • Distinguishes Staphylococcus aureus from coagulase negative Staphylococci (CoNS) in 20 minutes
  • Allows species ID to be reported with gram stain result
  • Works with samples taken directly from blood cultures: no sample preparation needed.
  • Ensure early, appropriate therapy for patients with S.aureus infections
  • Minimise the unnecessary use of vancomycin and broad-spectrum antibiotics

 

Download Staphylococcus QuickFISH Instructions for Use (IFU) Enquire about Staphylococcus QuickFISH

 

References

1. Forrest GN, Mehta S, Weekes E, Lincalis DP, Johnson JK, Venezia RA. Impact of rapid in situ hybridization testing on coagulase-negative staphylococci positive blood cultures. J Antimicrob Chemother. 2006 Jul;58(1):154-8

2. Ly T, Gulia J, Pyrgos V, Waga M, Shoham S. Impact upon clinical outcomes of translation of PNA FISH-generated laboratory data from the clinical microbiology bench to bedside in real time. Ther Clin Risk Manag. 2008 Jun;4(3):637-40.

About Sepsis Diagnostics

Sepsis is a potentially life-threatening condition caused by the presence of micro-organisms such as bacteria (bacteraemia) and fungi (fungaemia) in the bloodstream. Disease progression can be rapid so effective clinical intervention in these cases requires prompt diagnosis and identification of the responsible pathogen.

The QuickFISH system offers unprecedentedly fast (20 minute) species identification of pathogens directly from positive blood cultures, allowing the reporting of pathogen identification at the same time as the reporting of Gram stain results. Implementation of QuickFISH for sepsis diagnostics can deliver

  • Improved patient outcomes
  • Decreased financial outlay
  • Optimised antibiotic use
  • Decreased patient length of stay