Scientific Papers

scientific-papers-xpressfish-quickfish-1-700x200

You’ll find a list of relevant scientific papers concerning both the use of the QuickFISH system specifically, and the wider field of sepsis diagnostics on this page.

QuickFISH Scientific Papers, Posters and Publications

Key to Subject Matter
candida-quickfish-1 Candida
entrococcus-quickfish-1 Enterococcus
gramnegative-quickfish-1 Gram Negative
staphylococcus-quickfish-1 Staphylococcus
effect-on-patient-care-quickfish-1 Effect on Patient Care

 

Please see below for a selection of key publications that describe the performance and characteristics of the both the QuickFISH system specifically, and the AdvanDX PNA-FISH platform technology in general.

The most recent papers are listed first, followed by a list of the most relevant earlier publications. This page will be revised and updated as new publications appear.

Most Recent Scientific Publications

Calderaro A, et al.candida-quickfish-1 entrococcus-quickfish-1 gramnegative-quickfish-1 Publication features Staphylococcus

Comparison of PNA-FISH assays with culture based MALDI-TOF for the identification of bacteria and yeasts from blood cultures and yeasts from blood cultures and cerebrospinal fluid cultures.

Clin Microbiol Infect 2014.

Calderaro A, et al.

 

In this paper PNA-FISH assays were directly compared to MALDI-TOF for the identification of Staphylococci, Enterococci, Gram negative rods and yeasts from positive blood and cerebrospinal fluid cultures. A total of 956 cultures were tested. Key findings were that:

“The PNA-FISH assays allowed identification of bacteria and yeasts 1.3 days before MALDI-TOF and 2 days earlier in the case of polymicrobial infections.

The use of PNA-FISH would improve laboratory diagnosis of sepsis by providing species identification in a shorter time than culture-based MALDI-TOF identification”

It should also be noted that this study employed the first generation PNA-FISH: use of the QuickFISH technique would be expected to further enhance the observed effects.

 


Deck MK, et al. entrococcus-quickfish-1

Rapid detection of Enterococcus spp. Direct from blood cultures using Enterococcus QuickFISH method: a multicentre investigation – Diagnostic Microbiology and Infectious Disease.

Diagn Microbiol Infect Dis 2014; 78:338-42.

Deck MK, et al.

 

This publication discusses the application of the QuickFISH system to detect and identify Enterococcus species directly from positive blood cultures. This study found that:

QuickFISH offered the shortest turnaround time of any comparable test, including biochemical assays, amplified assays and MALDI-TOF.

QuickFISH provides results 1 day earlier than MALDI-TOF performed on an isolate”

 


Gorton RL, et al. candida-quickfish-1

Comparative analysis of Gram’s stain, PNA-FISH and Sepsityper with MALDI-TOF MS for the identification of yeast direct from positive blood cultures.

Mycoses. 2014 May 26. doi: 10.1111/myc.12205. [e-Publication ahead of print]

Gorton RL, et al.

 

This study aimed to evaluate three methods by which yeasts could be identified directly from blood cultures, one of which was the AdvanDX PNA-FISH method. After undertaking the comparison these authors found that:

“The aim of this study was to evaluate the utility of three rapid methods for the identification of yeast direct from blood culture. The most successful method was the PNA-FISH assay.

Contrasting Gram’s stain and MALDI-TOF MS, the success of the PNA-FISH Yeast assay was attributed to the minimal technical expertise necessary to perform the assay and the high specificity of the assay for its target molecule.

The financial burden of the Yeast PNA-FISH assay is not substantial and could ultimately lead to financial savings if clinical management can be influenced by the results”

It should also be noted that this study employed the first generation PNA-FISH: use of the QuickFISH technique would be expected to further enhance the observed effects.

 


Carretto E, et al. staphylococcus-quickfish-1

Comparison of the Staphylococcus QuickFISH BC test with the tube coagulase test performed on positive blood cultures for evaluation and application in a clinical routine setting.

J Clin Microbiol. 2013 Jan;51(1):131-5. doi: 10.1128/JCM.02103-12. Epub 2012 Oct 24

Carretto E, et al.

 

Here Caretto and co-workers compared the performance of the QuickFISH assay to that of a traditional coagulase assay to characterise Staphylococci. Their key findings were that:

“It has to be emphasized that the hands-on time of the QuickFISH Test is less than 5 min; the technique is easy, and the slides can be prepared by technicians after only a short training period. The introduction of the method in the laboratory routine therefore has a low implication in modifying the usual workflow.

The QuickFISH technology appears robust and reliable, with the major advantage of providing results in half an hour. It can be expected that this technique will be used routinely for all blood cultures”

 


Deck MK, et al. staphylococcus-quickfish-1

Multicenter Evaluation of the Staphylococcus QuickFISH Method for Simultaneous Identification of Staphylococcus aureus and Coagulase Negative Staphylococci Directly from Blood Culture Bottles in less than Thirty Minutes

J Clin Microbiol. 2012 Jun;50(6):1994-8. doi: 10.1128/JCM.00225-12. Epub 2012 Apr 4

Deck MK, et al.

 

This study set out to “determine whether the Staphylococcus QuickFISH method is robust and specific for identification of S. aureus and Coagulase Negative Staphylococci in the clinical microbiology laboratory setting.” The results of their assessment were that:

“Staphylococcus QuickFISH has a turnaround time (TAT) of S. aureus/CoNS identification simultaneously with Gram stain results.

Staphylococcus QuickFISH has the potential to significantly impact individual patient outcomes and overall bloodstream infection treatment costs by providing physicians with information to allow earlier implementation of targeted antibiotic therapies”

 


Parcell BJ and Orange GV gramnegative-quickfish-1 staphylococcus-quickfish-1 effect-on-patient-care-quickfish-1

PNA-FISH assays for early targeted bacteraemia treatment.

J Microbiol Methods. 2013 Nov;95(2):253-5. doi: 10.1016/j.mimet.2013.09.004.

Parcell BJ and Orange GV

 

These authors set out to determine whether the use of the Staphylococcus and Gram Negative Rod PNA-FISH assays would assist in the earlier targeting of treatment in patients with bacteraemia. The results of their study were:

“Start-up costs for PCR based detection methods or Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF) are high. We believe that PNA-FISH could be used as an economical alternative to these techniques by basing the use of certain PNA assays on Gram stain result. Identifying the organism earlier could lead to a reduction in inappropriate antibiotic use justifying this test.

In summary this study demonstrates that the tested PNA-FISH assays were highly sensitive and specific. They can be integrated into the laboratory as part of routine blood culture work without difficulty. We found that earlier targeted treatment would have been given in 14 cases (10.7% case overall) had the PNA-FISH results been available to the clinician”

 


Stone NR, Gorton RL, Barker K, Ramnarain P, Kibbler CC.candida-quickfish-1 effect-on-patient-care-quickfish-1

Evaluation of PNA-FISH yeast traffic light for rapid identification of yeast directly from positive blood cultures and assessment of clinical impact.

J Clin Microbiol. 2013 Apr;51(4):1301-2. doi: 10.1128/JCM.00028-13

Stone et al.

 

This study assessed the impact of the use of the PNA-FISH technology for the identification of the causative pathogen in cases of Candidaemia. The results suggested that the assay could have impacted antifungal therapy in a number of cases.

“Case note review suggested that had the PNA-FISH result been available to the clinician, there would have been no change in antifungal therapy in 32 cases (61.5%), a switch from caspofungin to fluconazole in 11 cases (21.2%), and a switch from fluconazole to caspofungin in 4 cases (7.7%).

This study finds the assay to be highly sensitive and specific, with a short turnaround time”

 

Other Scientific Publications

In addition to the most recent papers shown above , a large number of papers have appeared in previous years that describe the use of the system, either in the context of test performance, or its ability to deliver clinical benefits. These papers are listed below.

 

Harris DM, Hata DJ.candida-quickfish-1 entrococcus-quickfish-1 gramnegative-quickfish-1 Publication features Staphylococcus

Rapid identification of bacteria and Candida using PNA-FISH from blood and peritoneal fluid cultures: a retrospective clinical study.

Ann Clin Microbiol Antimicrob. 2013 Jan 7;12:2. doi: 10.1186/1476-0711-12-2

Harris DM, Hata DJ.

 


Ly T, Gulia J, Pyrgos V, Waga M, Shoham S.Publication features Staphylococcus Publication features Effects on Patient Care

Impact upon clinical outcomes of translation of PNA FISH-generated laboratory data from the clinical microbiology bench to bedside in real time.

Ther Clin Risk Manag. 2008 Jun;4(3):637-40.

Ly et al.

 


Forrest GN, Mehta S, Weekes E, Lincalis DP, Johnson JK, Venezia RA.Publication features Staphylococcus Publication features Effects on Patient Care

Impact of rapid in situ hybridization testing on coagulase-negative staphylococci positive blood cultures.

J Antimicrob Chemother. 2006 Jul;58(1):154-8. Epub 2006 Apr 24.

Forrest et al.

 


Forrest GN, Mankes K, Jabra-Rizk MA, Weekes E, Johnson JK, Lincalis DP, Venezia RA.Publication features Candida

Peptide nucleic acid fluorescence in situ hybridization-based identification of Candida albicans and its impact on mortality and antifungal therapy costs.

J Clin Microbiol. 2006 Sep;44(9):3381-3.

Forrest et al.

 


Alexander BD, Ashley ED, Reller LB, Reed SD.Publication features Candida

Cost savings with implementation of PNA FISH testing for identification of Candida albicans in blood cultures.

Diagn Microbiol Infect Dis. 2006 Apr;54(4):277-82. Epub 2006 Feb 8.

Alexander et al.

 


Hensley DM, Tapia R, Encina Y.Publication features Staphylococcus

An evaluation of the AdvanDx Staphylococcus aureus/CNS PNA FISH assay.

Clin Lab Sci. 2009 Winter;22(1):30-3.

Hensley et al.

 


González V, Padilla E, Giménez M, Vilaplana C, Pérez A, Fernández G, Quesada MD, Pallarés MA, Ausina V.Publication features Staphylococcus

Rapid diagnosis of Staphylococcus aureus bacteremia using S. aureus PNA FISH.

Eur J Clin Microbiol Infect Dis. 2004 May;23(5):396-8. Epub 2004 Apr 27.

González et al.

 


Oliveira K, Brecher SM, Durbin A, Shapiro DS, Schwartz DR, De Girolami PC, Dakos J, Procop GW, Wilson D, Hanna CS, Haase G, Peltroche-Llacsahuanga H, Chapin KC, Musgnug MC, Levi MH, Shoemaker C, Stender H.Publication features Staphylococcus

Direct identification of Staphylococcus aureus from positive blood culture bottles.

J Clin Microbiol. 2003 Feb;41(2):889-91

Oliveira et al.

 


Oliveira K, Procop GW, Wilson D, Coull J, Stender H.Publication features Staphylococcus

Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes.

J Clin Microbiol. 2002 Jan;40(1):247-51.

Oliveira et al.

 


P. Della-Latta, H. Salimnia, T. Painter, F. Wu, G.W. Procop, D.A. Wilson, W. Gillespie, A. Mender, B.S. CrystalPublication features Gram Negative

Identification of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in Blood Cultures: a Multicenter Performance Evaluation of a Three-Color Peptide Nucleic Acid Fluorescence In Situ Hybridization Assay.

J Clin Microbiol. 2011 Jun;49(6):2259-61.

Della-Latta et al.

 


Morgan M, Marlowe E, Della-Latta P, Salimnia H, Novak-Weekley S, Wu F, Crystal BS.Publication features Gram Negative

Multicenter evaluation of a new shortened peptide nucleic acid fluorescence in situ hybridization procedure for species identification of select Gram-negative bacilli from blood cultures.

J Clin Microbiol. 2010 Jun;48(6):2268-70.

Morgan et al.

 


Søgaard M, Hansen DS, Fiandaca MJ, Stender H, Schønheyder HC.Publication features Gram Negative

Peptide nucleic acid fluorescence in situ hybridization for rapid detection of Klebsiella pneumoniae from positive blood cultures.

J Med Microbiol. 2007 Jul;56(Pt 7):914-7.

Søgaard et al.

 


Søgaard M, Stender H, Schønheyder HC.Publication features Gram Negative

Direct identification of major blood culture pathogens, including Pseudomonas aeruginosa and Escherichia coli, by a panel of fluorescence in situ hybridization assays using peptide nucleic acid probes. J Clin Microbiol. 2005 Apr;43(4):1947-9.

Søgaard et al.

 


L. Hall, K.M. Le Febre, S.M. Deml, S.L. Wohlfiel, N.L. WengenackPublication features Candida

Evaluation of the Yeast Traffic Light(R) PNA FISH; Probes for Identification of Candida species from Positive Blood Cultures.

J Clin Microbiol. 2012 Jan 11.

Hall et al.

 


Shepard JR, Addison RM, Alexander BD, Della-Latta P, Gherna M, Haase G, Hall G, Johnson JK, Merz WG, Peltroche-Llacsahuanga H, Stender H, Venezia RA, Wilson D, Procop GW, Wu F, Fiandaca MJ.Publication features Candida

Multicenter evaluation of the Candida albicans/Candida glabrata peptide nucleic acid fluorescent in situ hybridization method for simultaneous dual-color identification of C. albicans and C. glabrata directly from blood culture bottles.

J Clin Microbiol. 2008 Jan;46(1):50-5. Epub 2007 Oct 31.

Shepard et al.

 


Wilson DA, Joyce MJ, Hall LS, Reller LB, Roberts GD, Hall GS, Alexander BD, Procop GW.Publication features Candida

Multicenter evaluation of a Candida albicans peptide nucleic acid fluorescent in situ hybridization probe for characterization of yeast isolates from blood cultures.

J Clin Microbiol. 2005 Jun;43(6):2909-12.

Wilson et al.

 


Rigby S, Procop GW, Haase G, Wilson D, Hall G, Kurtzman C, Oliveira K, Von Oy S, Hyldig-Nielsen JJ, Coull J, Stender H.Publication features Candida

Fluorescence in situ hybridization with peptide nucleic acid probes for rapid identification of Candida albicans directly from blood culture bottles.

J Clin Microbiol. 2002 Jun;40(6):2182-6.

Rigby et al.

 


Oliveira K, Haase G, Kurtzman C, Hyldig-Nielsen JJ, Stender H.Publication features Candida

Differentiation of Candida albicans and Candida dubliniensis by fluorescent in situ hybridization with peptide nucleic acid probes.

J Clin Microbiol. 2001 Nov;39(11):4138-41.

Oliveira et al.